Peptide Library Design

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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A synthetic peptide library is a collection of randomly synthesized small peptides of specific length using chemical synthesis methods. When synthesizing a peptide library, 20 natural amino acids can be used as building blocks, and the library capacity is determined by the number of amino acids (n) that make up a specific length peptide segment, which can be simply calculated as 20ⁿ. If a dipeptide library is constructed, there are 20² combinations, and a tripeptide library has 20³ combinations, and so on. The amino acids of the construction unit can not only utilize natural amino acids, but also introduce non natural amino acids such as D-amino acids, organic small molecules, etc., in order to construct peptide libraries with specific structures and functions. In 1991, Lam et al. first reported on Natrue a pentapeptide library synthesized using resin beads as carriers. In the peptide library, each resin bead is coupled with only one type of pentapeptide, and these pentapeptides can bind to corresponding proteins. Specific proteins such as antibodies, ligands, enzymes, etc. labeled with enzymes, fluorescein, and isotopes are used as probes. After interacting with the peptide library, resin beads containing a specific pentapeptide can be screened and isolated from the library based on these markers. By sequencing the peptide segments on them, active peptides can be obtained.The common methods for constructing peptide libraries in chemical synthesis include: mixing averaging method (one bead, one peptide), iterative unfolding method, position scanning method, photo controlled localization synthesis method, tea bag method, multi needle method, and fiber carrier method.

Compared with biosynthetic peptide libraries, the advantages of chemically synthesized peptide libraries are:

• In addition to natural L-amino acids, the amino acids that make up peptide segments can also be non natural amino acids, such as D-amino acids N-methylated amino acids and glycosylated amino acids.
• When constructing a peptide library, different secondary structures can be included, such as cyclic peptides, α-spiral and β-folding.
• The screening method of chemically synthesized peptide libraries has the characteristics of high efficiency, trace amount, and high automation, and the optimal sequence can be directly obtained after screening.

Our technical and applications support team can offer advice on library design and experimental set up and analysis. Relating to design, we can advise on the best overlap or offset of peptides to be used in your target application, and then using your full-length protein sequence, we can generate the list of peptides for your library. Assistance with experimental set-up and analysis is available for applications such as ELISPOT, cell culture, intracellular cytokine staining and flow cytometry.

The CreativePep^TM Peptide Library technology can be used for a number of different types of library:

• Overlapping peptide library

• Positional Scanning Library

A selected position or positions in a peptide sequence are each systematically replaced with different amino acids in order to determine the preferred amino acid residues at these positions, measured by corresponding increases in activity.

Synthetic peptide library screening services

Cyclic peptide libraries screening

• Construction of cyclic peptide libraries

A cyclic peptide library is composed of multiple peptides with cyclic structures. These cyclic peptides have significant applications in biology, such as drug molecules, enzyme inhibitors, receptor ligands, etc. Due to their stable three-dimensional structures and resistance to enzymatic degradation, cyclic peptides play an essential role in drug design and screening.

Design of cyclic peptide sequences: Design various cyclic peptide sequences based on the binding or active sites of the target protein using computer-aided design (CAD) and bioinformatics tools.

Synthesis of cyclic peptides: Synthesize linear peptide chains using solid-phase peptide synthesis (SPPS) and then cyclize the linear peptide chains through chemical methods.

Purification and identification: Purify and identify the synthesized cyclic peptides using high-performance liquid chromatography (HPLC) and mass spectrometry (MS) to ensure the purity and correct structure of the cyclic peptides.

Construction of cyclic peptide library: Combine the purified and identified cyclic peptides to form a library containing a large number of different cyclic peptides.

• Cyclic peptide library screening services

The cyclic peptide library screening service aims to find cyclic peptides with high affinity and specificity for target molecules (such as proteins, enzymes, or receptors) from a large number of cyclic peptides. The screening steps are as follows:

• Preparation of target protein: Express and purify the target protein to ensure its activity and correct folding.
• Screening techniques

Yeast display: Display the cyclic peptide library on the surface of yeast cells and use fluorescence-activated cell sorting (FACS) to screen yeast cells that bind to the target protein.

Phage display screening: Display the cyclic peptide library on the surface of phages and use ELISA or other detection methods to screen phages that bind to the target protein.

Surface plasmon resonance imaging(SPRi): Use SPRi technology to screen cyclic peptides in the library that have high affinity for the target protein.

• Validation and optimization: Measure the affinity, analyze the structure, and verify the function of the screened cyclic peptides. Optimize their structure if necessary to improve activity and stability.

Linear peptide libraries screening

• Construction of linear peptide libraries

A linear peptide library consists of multiple peptides with different sequences. These peptides have extensive diversity and are widely used in antibody generation, enzyme inhibitor screening, vaccine development, and other fields.

Design of linear peptide sequences: Design a linear peptide library with different amino acid sequences based on the characteristics and research purposes of the target molecules.

Synthesis of peptides: Synthesize linear peptides in bulk using SPPS techniques. During synthesis, various modifications can be introduced to improve the stability or activity of the peptides.

Purification and Identification: Purify and identify the synthesized linear peptides using HPLC and MS techniques.

Construction of linear peptide library: Combine the purified linear peptides to form a library containing a large number of different sequences.

• Linear peptide library screening services

The linear peptide library screening service aims to find linear peptides with high affinity and specificity for target molecules. The screening steps are as follows:

• Preparation of target protein: Express and purify the target protein.
• Screening techniques

ELISA screening: Use enzyme-linked immunosorbent assay (ELISA) to screen linear peptides that bind to the target protein.

Peptide microarray technology: Print the linear peptide library on slides and use fluorescent labeling to detect peptides that bind to the target protein.

High-throughput screening (HTS): Use automated platforms for high-throughput screening to quickly identify linear peptides that bind to the target protein.

• Validation and optimization: Further measure the affinity and verify the function of the screened linear peptides, optimizing their structure if necessary.

Why choose Creative Peptides?

• Comprehensive peptide libraries

We offer a number of different types of library, providing extensive diversity and coverage for various screening needs. Our libraries are meticulously designed and synthesized to ensure high-quality and functional peptides.

• Advanced screening technologies

We utilize cutting-edge technologies such as phage display and surface plasmon resonance imaging (SPRi) for effective and precise screening. High-throughput screening (HTS) and peptide microarray platforms enable rapid and large-scale identification of peptide hits.

• Customization and flexibility

Our services are highly customizable to meet the specific requirements of different projects.

We offer tailored peptide design and synthesis, ensuring that our libraries are optimized for your target proteins or research goals.

• High-quality synthesis and purification

Utilizing state-of-the-art solid-phase peptide synthesis (SPPS) techniques, we ensure high purity and accuracy in our peptide products. Rigorous purification processes, including HPLC and MS, guarantee the quality and integrity of our peptides.

• Expert team and support

Our team consists of experienced scientists and researchers with deep expertise in peptide chemistry, molecular biology, and drug discovery. We provide comprehensive support throughout the screening process, from initial consultation to post-screening analysis and optimization.

• Innovative solutions and continuous improvement

We are committed to innovation and continuously improving our methods and technologies to deliver the best possible results. Our R&D efforts focus on enhancing the efficiency and effectiveness of our screening services, ensuring we remain at the forefront of the industry.

• Cost-effective and timely delivery

We offer competitive pricing without compromising on quality, making our services accessible to a wide range of clients. Our streamlined processes and robust infrastructure ensure timely delivery of results, helping to accelerate your research and development timelines.

FAQ

1. What types of peptide libraries do you offer?

We offer both cyclic and linear peptide libraries. Our cyclic peptide libraries provide stable structures with enhanced resistance to enzymatic degradation, while our linear peptide libraries offer extensive sequence diversity suitable for various applications.

2. What are the applications of your peptide library screening services?

Our services are ideal for drug discovery, enzyme inhibition studies, receptor-ligand interaction analysis, vaccine development, and antibody generation. They can also be used for biomarker discovery and functional genomics studies.

3. Can you customize peptide libraries for specific projects?

Yes, we offer highly customizable services to meet the unique requirements of different projects. We can design and synthesize peptide libraries tailored to your specific target proteins or research objectives.

4. How long does the screening process take?

The duration of the screening process depends on the complexity and scale of the project. Typically, it can range from a few weeks to a few months. We provide detailed timelines during the initial consultation.

5. What information do you need from clients to start a project?

We require detailed information about your target molecule, the desired application, any specific requirements for the peptide library, and your project timeline. This helps us design and execute the screening process effectively.

6. What are the costs associated with your screening services?

The cost of our services varies depending on the specific requirements and scale of the project. We offer competitive pricing and work closely with clients to provide cost-effective solutions without compromising on quality.