Peptide Nucleic Acid Synthesis Service

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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PNAs, or peptide nucleic acids, is an analogue of oligonucleotide in which sugar-phosphate backbone has been replaced by a polyamide chain. Due to the unique backbone of PNA, it can be used in same applications as conventional DNA/RNA. Comparing with DNA, PNAs are neutral in charge, chemically more stable and resistant to DNases and proteases, as well as remaining high sequence specificity, binding activity and lower salt sensitivity. PNAs have been widely used in molecular diagnostics and therapeutics, such as fluorescent in situ hybridization (Fish), single nucleotide polymorphisms (SNPs) detection and antisense therapy.

Advantages of PNA Unique Backbone

• PNA binds to DNA/RNA with higher affinity and greater specificity than conventional oligonucleotides
• PNA is more stable than DNA or RNA as it is resistant to nucleases and proteases
• PNA can be hydridized at lower salt concentrations than DNA or RNA which leads to higher stringency
• PNA makes ideal reagents for antisense and antigene use, probes for molecular diagnostics, & microarrays

Applications of Peptide Nucleic Acid (PNA)

• Microarrays and biosensors
  PNA microarray combined with PCR could detect genetically modified organisms.
• PNA FISH Probes
  In fluorescent in situ hybridization (FISH), short probes can be difficult to detect (owing to insufficient hybridization and low levels of labelling), while longer probes can induce non-specific background fluorescence. In PNA-FISH, shorter PNA probes can be used (because of the higher specificity of PNA), resulting in less background noise. The fluorescent dye-conjugated PNA can bind to DNA or RNA quickly, even under low salt.
• PCR Blockers
  PNA probes have no direct interaction with DNA polymerase but PNAs can terminate the elongation of oligonucleotide primers by binding to the template or competing with the primers. Moreover, PNA-DNA chimeras can be recognized by the DNA polymerase and can thus be used as primers for PCR reactions. The high affinity binding of PNAs has also been used for detecting single base pair mutations by PCR. This strategy, named PNA directed PCR clamping, uses PNAs to inhibit the amplification of a specific target by direct competition of the PNA targeted against one of the PCR primer sites and the conventional PCR primer.
• PNA miRNA Inhibitors
  Compare to DNA/RNA, PNA has more strongly affinity when bind to complementary RNA. The character make PNA can be used as a miRNA inhibitors. Moreover, PNA miRNA inhibitors are conjugated to cell penetrating peptide (CPP), allowing them enter to cells without the need of transfection reagents.
• Antisense and Antigene Drugs
  PNA can bind to a complementary sequence of mRNA and change its function. PNA can break up DNA duplex and form PNA/DNA triplex or double duplexes without denaturing the DNA duplex. It can also inhibit the transcription and translation of target genes. Besides, PNA can improve the cellular delivery of PNA by binding of cell penetrating proteins (CPP). It has high sequence specifity for target in gene sequence.
• Double Strand DNA Invasion and Capture
  Because of its uncharged polyamide backbone, PNA can hybridize to negatively charged DNA or RNA without electrostatic repulsion.

We offer the many custom PNA synthesis services:

• PNA synthesis by automated solid-phase methods, as with DNA and RNA oligonucleotide synthesis.
• PNA Scales from 5mg to mutli-gram
• PNAs from 3 to 100 monomers
• PNA-Peptide conjugations
• PNA-Protein conjugations
• Specialized PNA modifications
• PNA libraries
• PNA Arrays
• PNA Purification, PNA purities from crude to >98%

Competitive Advantages

• Highly Customizable: Flexible synthesis scales, various modification types, and diversified delivery forms.
• Excellent Stability: Each batch of synthetic products is strictly purified by HPLC in the RNase-free environment to avoid RNase contamination in the whole process.
• High quality: Reliable lab report with timely updates. A RP-HPLC trace and Mass Spec analysis are included.
• Technical Support: Professional team provides you with comprehensive pre-sale consultation, order design, and after-sales service to ensure the products deliver smoothly.
• Cost-Effective: Competitive prices and short turnaround time.

Creative Peptides can provide a number of PNA related products, such as Gamma PNA or Fmoc/Boc PNA Monomers etc.