Custom Locked Nucleic Acid Synthesis

Our global business capability is designed to provide you with the highest quality locked nucleic acid oligonucleotides prepared according to your exact specifications. With many years of oligonucleotide synthesis expertise, Creative Peptides is proud of our cooperation with you from early research to commercial launch.

What is Locked Nucleic Acid?

Locked nucleic acid is a kind of RNA analogues based on non-deoxyribose, in which the 2'-O on the ribosome ring is connected to 4'-C, so that the five-membered ring is "locked" in the ideal Watson-Crick three-dimensional binding conformation. Therefore, Locked nucleic acid obeys stronger base complementary pairing, greater base accumulation force and better hybrid stability, and will not be recognized and degraded by nuclease in tissue cells. When a locked nucleic acid monomer is added to the nucleic acid chain, the temperature (T_M) value of the double-stranded hybrid can be increased by 2-8℃. Locked nucleic acid increases the differentiation of base mismatch, which can distinguish the difference of single base, and can make the oligos containing locked nucleic acid maintain a high T_M value at a shorter length.

Advantage of Locked Nucleic Acid

• High stability in vivo and in vitro.
• Locked nucleic acid can detect single nucleotide mismatch.
• A remarkable distinction between the miRNA family.
• Locked nucleic acid has ability to combine efficiently with RNA and DNA.
• High thermal stability and hybridization specificity.
• Excellent antisense inhibition on small RNA targets in vivo.
• Gene quantification and allele identification are more accurate.
• Locked nucleic acid can improve assay sensitivity and specificity.
• Reliable detection of all miRNA sequences, regardless of GC content.
• Compared with DNA and RNA probes, the target specificity is higher.
• Excellent testing from challenging samples such as biofluids and FFPE samples.
• Better mismatch discrimination in Single nucleotide polymorphisms (SNPs) genotyping assays.
• The specificity and sensitivity of non-coding RNA and other small RNA molecules are greatly improved.

Application of Locked Nucleic Acid

• Single nucleotide polymorphisms (SNPs) detection
• Gene copy assay
• Allele identification
• Pathogen detection
• Multiple determination
• Gene expression analysis
• Quantitative determination of virus content
• Mixed with all available qPCR detection chemicals

Our Services

• One-stop customized locked nucleic acid synthesis services from primer design and synthesis to final result delivery.
• Different modifications for all customized locked nucleic acid, such as phosphorylation, thiolation, modified bases, linkages, etc.
• Flexible design of LNA/DNA and LNA/RNA hybrid oligonucleotides.
• Our sequences are dual-HPLC purified and MS verified.

References

1. Vester, B., & Wengel, J. (2004). LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA. Biochemistry, 43(42), 13233-13241.
2. GrÜnweiler, A., & Hartmann, R. K. (2007). Locked nucleic acid oligonucleotides. BioDrugs, 21(4), 235-243.
3. Hagedorn, P. H., Persson, R., Funder, E. D., Albæk, N., Diemer, S. L., Hansen, D. J., ... & Koch, T. (2018). Locked nucleic acid: modality, diversity, and drug discovery. Drug Discovery Today, 23(1), 101-114.
4. Braasch, D. A., & Corey, D. R. (2001). Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA. Chemistry & biology, 8(1), 1-7.