IntraCellular Delivery System(ICDS™)

Creative Peptides has launched a novel ICDS^TM-NH2-kit. It could be used to facilitate the transfer of peptides or proteins as well as antibodies across cellular membranes in the cell-based assay.

Description

Therapeutic protein drugs are an important class of medicines most in need of novel therapies. Recently approved recombinant protein therapeutics have been developed to treat a wide variety of clinical indications, including cancers, autoimmunity/inflammation, exposure to infectious agents, and genetic disorders. However, the failure of proteins to penetrate mammalian cells or target tumor cells restricts their value as therapeutic tools in a variety of diseases. Unlike small molecules, a peptide or a protein is normally difficult enter into intact cells due to the cell membrane's semipermeability nature.

Creative Peptides offer ICDS^TM-NH2-kit containing good carriers that can easily cross cell membranes. ICDS^TM-NH2-kit considered as useful tools to assist the transshipment of peptide or protein from outside to the inside of cells. It provides a convenient way to deliver your bioactive proteins into cells by conjugating them with ICDS^TM peptides. This ICDS^TM-NH2-kit can be used with a wide range of molecule up to 150kDa. In order to verify its effectiveness, Creative Peptides’ scientists have tested ICDS^TM-NH2-kit using a wide variety type of cells, such as SkBr3, HEK293 and CHO, and nearly all of the tests have the positive results.

How does it work?

ICDS^TM-NH2-kit mainly composed of ICDS^TM peptides and linker, can assemble your protein with ICDS^TM peptides to form a conjugate which can flexible transfer across the cell membrane. ICDS^TM-NH2-kit is highly and specifically adaptable to many possible applications, giving your research great advantages.

Protocol at a glance

• Add ICDS^TM linker solution to your protein sample and mix for 30 minutes and centrifuge for 10 minutes @ 10,000 g.
• Add PBS buffer and centrifuge for 10 minutes @ 10,000 g (2x).
• Add ICDS^TM peptides for 2h at room temperature and centrifuge for 10 minutes @ 10,000 g.
• Move to a new filtration tube and add PBS buffer centrifuge for 10 minutes @ 10,000 g (2x).

Application

• Protein/peptide-base vaccine
• Drug delivery
• Alteration of biological activity in cells/tissues

If you foresee a possible future use for your research in a clinical setting, we can help. Contact us to customize your ICDS^TM service today!