Peptide Drug 3D Cell Efficacy Test Platform

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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At the forefront of biomedical innovation, peptide drugs have reshaped the treatment landscape with their exceptional specificity, high potency, and multipotency, yet ensuring that these novel therapies are both safe and effective remains a major challenge in drug development. Traditional two-dimensional cell models are often ineffective in predicting clinical responses because they cannot fully simulate the complex cell interactions and the subtle structure of the microenvironment in vivo. To this end, the 3D cell efficacy test platform launched by Creative Peptides breaks through the above limitations and builds a more suitable physiological test scene for the evaluation of peptide drugs.

Fig. 1 (A) Schematic diagram of the sample dispensing and dispensing uniformity performance evaluation process using the proposed 3D-Cell spotter. (B). 384-Pillar plate photo and experimental procedure for 3D cell culture and drug efficacy test. (C). Quantitative analysis of drug efficacy using an automated optical scanner and image analysis software. (Lee, S. Y.; 2022)

Transformative implications of 3D cell culture

The introduction of 3D cell efficacy detection technology significantly enhances the physiological relevance and prediction accuracy of drug screening compared with the traditional 2D model, and its core advantages are reflected in the following aspects:

A leap forward in physiological authenticity: 3D culture systems facilitate the formation of cells into spheres or aggregates that more closely resemble their physiological state, facilitating natural interactions between cells and the cell-to-extracellular matrix (ECM), accurately reflecting gene expression and protein profiles, resulting in more accurate drug response models.

Physiological simulation of cell behavior: Compared with 2D, cells in 3D environment proliferate at a lower speed, forming a spherical or aggregated form similar to the internal environment, and going through a variety of cell cycle stages. This more realistic physiological state also simulates conditions such as oxygenation and nutrient restriction in the body, profoundly affecting the evaluation of drug sensitivity and resistance.

Precise analysis of drug response: Studies have shown that, as Rasheena Edmondson's team found, colon cancer HCT-116 cells in 3D culture show different resistance to certain anticancer drugs than in 2D environments, underlining the unique value of 3D culture in revealing the true mechanism of action of drugs and drug resistance research.

Win-win for ethics and efficiency: In the initial stage of drug development, the application of 3D cell culture technology greatly reduces the dependence on animal experiments, reflecting the respect and progress of scientific research ethics.

Technology integration and innovation: Combining cutting-edge technologies such as high-throughput imaging, microfluidic technology, and tissue engineering, the 3D cellular efficacy platform further advances the accuracy and efficiency of drug evaluation, opening up unprecedented perspectives and possibilities for drug development.

3D cell efficacy test platform at Creative Peptides

Our 3D cell efficacy evaluation platform represents a revolutionary breakthrough in biomedical research, integrating cutting-edge 3D culture technology with comprehensive analytical capabilities to provide an efficient and accurate in vitro testing environment for drug discovery and development.

Innovative 3D culture technology matrix

We have adopted a variety of cutting-edge 3D cell culture systems to meet a wide range of research needs.

Spherical aggregates: Mimic the microenvironmental structure of tumors and other tissues, showing the natural state of cell clusters.

Organoid models: miniature organ models derived from stem cells that accurately replicate the complexity of organ structure and function.

Functional hydrogels: Dynamic matrices that provide cell support to create a highly adjustable experimental scenario for cell-to-matrix interaction studies.

Microfluidic chip technology: The use of microfluidics to accurately regulate fluid dynamics, simulate blood circulation and tissue material exchange in the body, and improve the physiological relevance of the experiment.

High content image analysis system

Advanced imaging technology is used to analyze the fine structure of 3D cell cultures in all aspects.

Real-time live cell imaging: Dynamic tracking of cell behavior and drug effects.

Fluorescence and confocal microscopy: Capture high-resolution images of cellular activity and interactions for greater understanding.

Precision quantitative evaluation method

Cell viability and proliferation assay: ATP detection, MTT reduction and BrdU incorporation techniques were used to accurately quantify cell activity and proliferation.

Analysis of cell death mechanism: apoptotic and necrotic pathways were distinguished by flow cytometry and Caspase activity test.

Evaluation of cell migration and invasion ability: The regulation of peptide drugs on cell migration behavior was evaluated using scratch and transpore migration experiments.

Customized Extracellular matrix (ECM) configuration

ECM formulations are tailored to specific research needs to create a microenvironment that better fits the actual situation in the body, significantly improving the physiological relevance and predictive accuracy of drug testing.

Peptide drug 3D cell efficacy test services

As a professional biopharmaceutical service provider, we focus on the field of 3D cellular drug screening, relying on advanced research and development and animal testing facilities, providing a variety of services.

High-quality 3D cell model construction: high-purity cell lines are obtained by single cell amplification technology to lay a solid foundation for drug screening.

High-throughput drug screening platform: Large-scale screening using 3D cell models to accelerate the identification of potential drug candidates.

Accurate IC₅₀ assay service: Determine the median inhibitory concentration and minimum effective concentration of a drug, and provide toxicological evaluation reports simultaneously.

Organ-specific toxicity assessment: 3D hepatocyte and cardiomyocyte models are used to accurately predict hepatotoxicity and cardiotoxicity of drugs, providing key data for safety assessment.

Personalized test program design: Customized development of 3D cellular drug screening strategies according to customer needs.

Professional data analysis and report preparation

Our team of senior experts provide detailed data analysis and comprehensive reports to help clients understand research findings and provide strong support for scientific decisions on candidate peptide drugs.

FAQ

1. What types of cells can be used in the 3D cell efficacy test platform?

Our platform supports a diverse array of cell types, including but not limited to, cancer cells, stem cells, primary cells, and other specialized cell lines. This versatility allows us to model different disease states and evaluate drug efficacy across various biological contexts.

2. How does the 3D cell culture method improve the accuracy of drug efficacy testing compared to 2D culture methods?

The 3D cell culture method better mimics the natural cellular environment by allowing cells to grow and interact in three dimensions, similar to how they would in the human body. This leads to more physiologically relevant data, improves the prediction of drug responses, and reduces the risk of false positives/negatives that commonly occur in 2D cultures.

3. What kind of endpoints can be measured using this platform?

Our platform can measure a variety of cellular endpoints, including cell proliferation, apoptosis (programmed cell death), migration, invasion, and drug uptake. These measurements provide comprehensive insights into the therapeutic potential and mechanism of action of your peptide drug.

4. How long does it typically take to complete a 3D efficacy test?

The duration of a 3D efficacy test can vary depending on the complexity of the experiment and the specific requirements of the client. Generally, most tests can be completed within 2-4 weeks. However, larger or more complex studies may take longer.

5. Can the platform be used to test combination therapies involving peptide drugs?

Yes, our platform is fully capable of assessing the efficacy of combination therapies. We can evaluate how different peptide drugs interact with each other and with other types of therapeutic agents to determine synergistic, additive, or antagonistic effects.

6. Is it possible to customize the experimental design to fit specific research needs?

Absolutely. We offer tailored experimental designs to meet the unique requirements of your research. Our team of experts will work closely with you to develop a protocol that addresses your specific objectives and desired outcomes.

7. What kind of data and reporting will be provided at the conclusion of the testing?

Upon completion of the testing, clients will receive a comprehensive report that includes all raw data, detailed analysis, interpretation of results, and any relevant visual aids such as graphs or histograms. We also offer consultation services to help you understand the results and plan subsequent steps.

Reference

1. Lee, S. Y.; et al. Optimization of 3D-aggregated spheroid model (3D-ASM) for selecting high efficacy drugs. Scientific Reports. 2022, 12(1): 18937.