Custom Peptide Synthesis

Creative Peptides has long-term peptide synthesis experience and rich expertise. We provide high-quality customized peptide synthesis services, from bulk API peptides, high-throughput library peptides, cosmetic peptides, to array peptides, antigen peptides, and other complex or abnormal peptide sequences.

Applications of Peptide Synthesis

Synthetic peptides play a central role in MS-based protein discovery, characterization, and quantitative analysis, especially those proteins that serve as early disease biomarkers.

In addition, synthetic peptides also have important application prospects in clinical diagnosis and disease treatment.

Research fields

Peptide Synthesis Services

We can synthesize peptides of 200 amino acids and above. Longer peptides can be prepared by recombinant peptide technology.

• Free peptide consultation and design
• Custom peptides on demand
• Ultra long peptides
• Specialty peptide modifications
• Mass spectrometry and HPLC chromatogram to confirm purity and identity
• Provided as a lyophilized powder to minimize oxidation

Methods & Quality

Our custom peptide synthesis services range from research grade to many API peptides. Creative Peptides is proficient in different synthesis methods to meet different needs.

• Liquid Phase Peptide Synthesis is used for the synthesis of short peptides (such as dipeptides).
• Solid Phase Peptide Synthesis is used to prepare biologically active peptides including long, difficult, and hydrophobic peptides.
• Biosynthesis provides many peptide modifications and customized peptides for diagnostic and therapeutic applications.

Peptide synthesis quality control

After peptide synthesis, each peptide is routinely analyzed by LC-MS, MALDI-MS, or HPLC to confirm the identity and quality of the customized peptide.

• MALDI-MS
  All peptides are analyzed by MALDI-MS to determine the molecular weight of the synthesized peptides.
  
• HPLC
  MALDI is an excellent method to determine the presence of the desired product, but it is not sufficient to determine purity. So, all non-crude peptides must be analyzed by reverse-phase HPLC (RP-HPLC) to determine purity. Other QC analysis, such as amino acid analysis, NMR, CE, and peptide content determination can be performed to confirm the quality of customized peptides.

• Extended quality control (optional)
  Chromatography (HPLC, TLC, GC, IC)
  Chromatography combined with mass spectrometry (LC-MS / ESI)
  Spectroscopy (UV-Vis, IR, NMR)
  Metal analysis (AAS, ICP, ICP-MS)
  Elemental analysis
  Titration (acid-base titration, Karl Fischer titration)
  Amino acid analysis
  Peptide sequencing
  

Our advantage

• Consistent results: The success rate of delivering high-quality peptides stored in argon stabilizers is> 99%
• Unlimited: short or long peptides, chemical or recombinant peptide technology
• Large number of conjugations, modifications, and labeling
• Quality control: all peptides are analyzed by MS and HPLC
• Fast turnaround
• Free peptide design tool
• Competitive price

In addition, we also provide peptide modification, conjugation and analysis services.

Modifications & Labels

Creative Peptides also has extensive experience in the design and production of modified peptides. We provide a variety of peptide modifications, labeling, and bioconjugation with other biomolecules, including

• Peptide N-Terminal Modification
• Peptide C-Terminal Modification
• Peptide PEGylation
• Biotinylated Peptides
• Fluorescence and Dye Labeling Peptides
• Post-translational Modification
• Custom Labeling
• Peptide Tags
• Unusual and Non-natural Amino Acids Modification

Peptide Conjugations

Creative Peptides provides one-stop peptide solutions, from peptide synthesis, modification, labeling and conjugation to a wide range of biopolymers and surface attachments.

Our peptides conjugation services include, but are not limited to

• Gold/Silver Nanoparticles Conjugation Service
• Peptides-Carraier Protein Conjugation
• Peptide-oligonucleotide Conjugation
• Peptide-enzyme Conjugation
• Peptides-Metal Chelates Conjugation
• NHS-Activated Peptide Conjugation Magnetic Beads
• Peptides-Imaging Agents Conjugation
• Peptides-Fatty Acids Conjugation
• Peptide-small-molecule Conjugation
• Peptide-Antibody Conjugation
• Peptide-siRNA Conjugation
• Peptide-DNA Conjugation
• Peptide-RNA Conjugation

Analysis Service

In addition, we also provide a variety of optional peptide analysis, including

• Surface Plasmon Resonance Imaging (SPRi)Service
• Epitope Mapping Services
• Peptide Characterization
• Peptide Library and Array
• Biological Testing
• Chemical & Physical Analyses