Peptide Acetylation

Acetylation refers to the substitution of hydrogen by acetyl group in organic molecules with oxygen, nitrogen, carbon, sulfur atoms. Commonly used acylation agents are carboxylic acylation agents, carboxylic ester acylation agents, anhydride acylation agents and acyl chloride acylating agents. Acyl groups are the groups remaining after removal of hydroxyl groups from organic acids, inorganic acids, sulfonic acids, etc. Chemically synthesized peptides often carry free amino groups and free carboxyl groups. The sequence of peptide often represents the sequence of female protein.

In order to be closer to female protein, the end of peptide often needs to be closed, that is, N-terminal acetylation and C-terminal amidation, which can reduce the total charge of peptide and the solubility of peptide. Peptides can also mimic its original state of α-amino and carboxyl groups in maternal proteins. Acetylation modification of peptides is a reversible post-translational modification process that can be expressed in both eukaryotic and prokaryotic cells. In the past few decades, many researches have been carried out on the acetylation modification of amino acids.

Creative Peptides offers custom production of peptides containing acetylated residues. The incorporation of acetylated Lysine residues is in most cases straight forward. Nevertheless, when the objective is to obtain high quality material, we know from experience that you need the better reagents, a sound synthesis methodology, and quality control to match it.

We offer excellent acetylated peptides that:

• Modified sites can be easily identified by mass spectrometry
• Allows to count the number of lysine residues
• Removes the positive charge on the N-terminal of peptides, thus mimicking natural proteins
• Increases peptide stability by preventing N-terminal degradation
• Can be expressed in both eukaryotic and prokaryotic cells

Every step of peptide synthesis is subject to Creative Peptides’ stringent quality control. Typical delivery specifications include:

• HPLC chromatogram
• Mass spec analysis
• Synthesis report
• Certificate of Analyses

Reference

1. Walsh, C. T., Garneau‐Tsodikova, S., & Gatto Jr, G. J. (2005). Protein posttranslational modifications: the chemistry of proteome diversifications. Angewandte Chemie International Edition, 44(45), 7342-7372.