Phage Display Peptide Library
* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).
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Phage display is one of the most powerful and widely used laboratory techniques for studying protein-protein, protein-peptide, and protein-DNA interactions. This technology is an in vitro screening technique. A large number of peptides or proteins are made into peptide (protein) library, and then fused with phage coat protein for expression. Peptide is displayed on the surface of virus particles, while the DNA encoding the peptide is located inside the virus particles. Using this relationship between the displayed peptide and its coding DNA, the required specific peptide/protein was quickly screened through multiple rounds of affinity panning, and then the phage was amplified and sequenced directly to find out the DNA sequence corresponding to the specific binding peptide/protein. Using phage display peptide library, billions of peptides can be quickly screened through a large number of different target molecules.
Creative Peptides has dedicated many years to the development and application of phage display technology. With our extensive experience, our scientists have developed multiple phage display-based platforms and tailored numerous peptide libraries to advance our global customers' research and project goals. We are proud to leverage our extensive experience and advanced platforms to provide the best service and the highest quality products to meet each customer's specific needs.
Phage display peptide library applications
In the past twenty years, phage peptide library has become the mainstream tool for peptide screening, and it has been widely used in mapping antigen epitopes, mapping protein-protein interactions and identifying peptide mimetics of non-peptide ligands. In addition, purified receptors, intact cells and tissue samples were used as target molecules for peptide library panning. In vitro and in vivo experiments, it was proved that bioactive peptides could be used as cell targeting or gene delivery agents. Targeting peptides identified using phage display technology may be useful for basic research and translational medicine.
Our phage display peptide library services
At Creative Peptides, we offer comprehensive phage display peptide library construction services. Our capabilities include creating various types of peptide libraries, customized to meet the specific needs of each project. We are proficient in tailoring the most suitable phage display system for library construction, including M13, T4, T7, or lambda phages.
- Types of phage display peptide libraries: Linear peptide libraries, cyclic peptide libraries, random peptide libraries, target-specific peptide libraries.
- Our phage display peptide libraries boast a large capacity, ranging from 107 to over 1012 unique clones.
Phage display peptide library screening is a critical step in the selection of high-affinity binders. At Creative Peptides, we utilize proprietary protocols and tailored biopanning strategies to ensure successful screening outcomes. Our biopanning strategies include solid-phase screening, solution-sorting screening, cell-based screening, and in vivo and ex vivo screening. Our phage display peptide library screening services are designed to select peptides with extremely high affinity and is suitable for a variety of peptide sources. Creative Peptides also offers a range of in-house premade peptide libraries.
Hit identification
This stage involves separating individual phage clones after the screening process. After isolation, the clones are then amplified to identify the displayed peptides. This identification is achieved through a process known as DNA sequencing, which is a technique used to determine the precise order of nucleotides within a DNA molecule. Furthermore, bioinformatic analysis, which combines biology, computer science, information engineering, and mathematics to analyze and interpret biological data, is also used in this process.
Characterization of hits
The second step in this process is to validate the binding specificity and affinity of the identified peptides to the target. This validation is performed using various techniques such as surface plasmon resonance (SPR), Biacore, or ELISA tests. SPR is a detection method used in molecular biology and biochemistry to detect molecular interactions, Biacore is a biosensor device that utilizes SPR for the purpose of detecting molecular interactions, while ELISA tests use components of the immune system to detect the presence of antigens.
Peptide optimization
This stage includes further assistance in optimizing the identified peptides. Optimization in this context refers to improving the peptides' affinity (how strongly they bind to the target), specificity (how selective they are), stability, and other properties, depending on the specific needs of the research.
Production and purification
The final step in the process involves the production and purification of the selected peptides. This can be done at different scales with varying degrees of purity, depending on the research requirements. The peptides are synthesized (produced) and then purified to remove any impurities before they can be used for further research.
PTM specific peptide discovery
Post-translational modifications (PTMs) play a crucial role in regulating protein function and activity. Creative Peptides provides specialized services for the discovery of peptides specific to PTMs. These peptides can recognize modifications such as phosphorylation, glycosylation, methylation, acetylation, and ubiquitination, among others. Our PTM-specific peptide discovery services are essential for studying cellular signaling pathways, disease mechanisms, and biomarker identification.
Why choose Creative Peptides?
- Leading technology and rich experience
Our company has rich experience and leading technical strength in phage display technology. Through continuous research and innovation, we can ensure the efficiency, accuracy and reliability of phage display peptide library service.
- Qualcomm screening and fast iteration
Our phage display peptide library service adopts Qualcomm screening technology, which can screen a large number of peptides in a short time, so as to quickly find peptides with high affinity and specificity to specific targets. Through rapid iteration and optimization of screening conditions, we can continuously improve the efficiency and accuracy of screening to meet the diverse needs of customers.
- Diversity and flexibility
The phage display peptide library provided by us has high diversity and flexibility. We can customize peptide libraries with different scales, different sequence composition and modification methods according to the needs of customers to meet the needs of different research fields. At the same time, we also provide personalized service solutions, which are customized according to the specific needs of customers to ensure that the service content is highly compatible with the research objectives of customers.
We strictly control the quality of phage display peptide library service. From the construction and screening of phage library to the purification and identification of peptides, we follow strict operating procedures and quality standards to ensure the purity and quality of each polypeptide product. We also confirmed that the screened polypeptide has stable biological activity and good specificity through many times of verification and experimental verification.
- Comprehensive technical support and after-sales service
We provide customers with comprehensive technical support and consulting services. Our team of technical experts will provide customers with one-on-one technical guidance, answer the problems encountered by customers during use and provide targeted solutions.
Creative Peptides is committed to advancing scientific research through innovative phage display peptide library solutions. Our state-of-the-art technology, combined with our experienced team, ensures that we deliver high-quality services and products to meet the diverse needs of our global customers. Whether you are looking to construct a custom phage display peptide library or require high-affinity peptides for your research, Creative Peptides is here to support your scientific endeavors.
FAQ
1. What is phage display technology?
Phage display is a laboratory technique used to study interactions between proteins, peptides, and DNA by displaying the peptide of interest on the surface of a bacteriophage.
2. How does phage display work?
The gene encoding the peptide of interest is inserted into a phage coat protein gene, resulting in the display of the peptide on the phage surface. This allows for the screening and selection of binders from a library of phages.
3. What types of phage display peptide libraries can Creative Peptides construct?
We construct various types of peptide libraries, including linear peptide libraries, cyclic peptide libraries, random peptide libraries, and target-specific peptide libraries.
4. What phage display systems does Creative Peptides use?
We tailor the most suitable phage display system for each project, including M13, T4, T7, and lambda phages.
5. What is the library capacity and affinity offered by Creative Peptides?
Our libraries have a capacity ranging from 107 to over 1012 unique clones, with high affinities typically between 10-7 and 10-9.
6. What biopanning strategies does Creative Peptides employ for library screening?
We use solid-phase screening, solution-sorting screening, cell-based screening, and in vivo and ex vivo screening strategies.
7. Can Creative Peptides provide PTM-specific peptides?
Yes, we offer services for the discovery of peptides specific to post-translational modifications.
8. What are the applications of phage display technology?
Phage display technology is used for discovering high-affinity binders, mapping epitopes, identifying enzyme substrates, studying protein interactions, and developing therapeutic peptides.
9. Does Creative Peptides offer premade phage display peptide libraries?
Yes, we have a range of in-house premade peptide libraries available for immediate screening.
