Phage Display Peptide Library Screening

* Please kindly note that our products and services can only be used to support research purposes (Not for clinical use).

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Creative Peptides is committed to providing customers with high-affinity small molecule peptide pre-screening technology services, and providing customers with subsequent cellular functional verification of small molecule peptides (in vitro recognition and inhibition function verification of small molecule peptides, in vitro flow blocking function), in vivo functional verification (in vivo targeted inhibition function verification of small molecule peptides, signaling pathway blocking function verification), and the development of small molecule peptide drugs and other downstream research and development work to provide strong support. Creative Peptides has many years of project experience and experience in peptide library screening, and has established a complete peptide library screening system, which can provide customers with high-quality targets including proteins, peptides and various peptide library screening services for targets such as cells (various types of tumor cells, primary cells).

The peptide library screening service is a screening service based on phage display technology. It refers to using proteins, peptides, cells and other screening antigens as screening targets, and obtaining high-affinity targeted peptide sequences after three rounds of pressure screening. The peptide library established by Creative Peptides has a library capacity of up to 10⁸ and a pre-screening titer of up to 10¹³ phage display peptide particles/ml, which is sufficient to support the screening of peptides targeting various targets and meeting the downstream experimental needs of customers. Based on the peptide library screening technology platform, the peptide affinity can reach the mM-μM level, and a variety of screening methods are available (solid phase screening, magnetic bead screening, cell screening and animal in vivo screening). Our scientists will recommend the most suitable screening method based on the customer's screening targets and project needs to obtain high-affinity peptides to meet the customer's project needs. At the same time, Creative Peptides can also provide a variety of supporting downstream verification experiments including peptide affinity verification (EC50 verification, BLI affinity verification and SPR affinity verification), flow blocking verification, etc. Customers only need to provide screening targets and project needs, Creative Peptides' scientists provide one-to-one customized solutions based on customer needs, saving you valuable time.

Our phage display peptide library screening

Phage display peptide library screening is a critical step in the selection of high-affinity binders. At Creative Peptides, we utilize proprietary protocols and tailored biopanning strategies to ensure successful screening outcomes.

Fig. 1 Screen the peptide from the phage display library. (Zhang, K., 2022)

Based on the characteristics of peptides such as small molecular weight, low immunogenicity, low toxicity, high tissue permeability, and high stability, more and more researchers prefer to screen targeting peptides with a smaller molecular weight than nanobodies as new treatments. molecules and excellent drug carriers. Peptide library screening is currently widely used in small molecule peptide drug discovery, peptide vaccine research and development, biomarker discovery, cell receptor targeting functional peptide screening, cancer targeted therapy, cell signaling pathway research and other fields. It is currently a hot topic and preferred choice for targeted drug development research. In order to obtain more stable targeted peptides, the modification of peptide structures is also an urgent problem that needs to be solved, such as peptide cyclization, peptide modification.

Creative Peptides provides a variety of antigen screening (proteins, peptides, cells) and screening methods. Different screening methods are suitable for different screening antigens. For example, the molecular weight of peptides is small, and there are few epitopes that can be recognized by targeting peptides. Therefore, it is necessary to use magnetic bead screening methods to increase the freedom of screening antigens, in order to obtain high-affinity and high-specific targeting peptides to meet subsequent needs. To meet the needs of project research and development, our scientists will design the most appropriate peptide library screening method based on the antigen screening situation and the customer's downstream experiments.

Screening strategies

Solid-phase screening: Involves immobilizing the target on a solid surface and exposing it to the phage display library. Phages that bind to the target are isolated and amplified for further rounds of selection.

Solution-sorting screening: In this method, the target is in solution, allowing for the identification of binders that recognize the native conformation of the target.

Cell-based screening: A method in particular for cell surface antigen selection. It is a reliable approach to screen cell surface antigens with native conformation, especially when the antigen is hardly expressed as recombinant protein.

In vivo and ex vivo screening: These approaches involve screening phage libraries in living organisms or freshly isolated tissues, respectively, to identify binders that function in a biological environment.

Acceptable library

• Libraries from our customized library construction services
• Libraries provided by our clients
• Creative Peptides also offers a range of in-house premade peptide libraries. These libraries are readily available for immediate screening and can accelerate the discovery process for our customers
• Other commercialized libraries

Peptide library screening

Screening for blue and white spots: The default is 3 rounds of screening (liquid phase, solid-liquid phase, cell screening). Convenient blue and white shift screening system selects single clones for sequencing without ELISA verification. Deliver the peptide sequence, screened peptide sequence information (gene information, amino acid sequence information and sequencing raw data) and project report.

Peptide synthesis and affinity verification

• Peptide synthesis
• EC50 verification/BLI affinity verification/SPR affinity verification
• Deliver experimental reports and raw data

Why choose Creative Peptides?

• A variety of peptide libraries can be selected: linear 7 peptide library, linear 12 peptide library, linear 15 peptide library, cyclic 7 peptide library.
• A variety of screening methods can be selected: solid phase screening, magnetic bead screening, cell screening, animal in vivo screening.
• A variety of screening targets can be selected: proteins, peptides, cells (various tumor cells, primary cells).
• Mature technology platform: the affinity of peptides obtained by screening is generally at the level of mM-μM.
• Matching downstream verification experiments: affinity verification (including EC50, BLI and SPR affinity verification), streaming blocking verification.
• One-to-one personalized solution customization to meet the needs of various customers' scientific research projects.
• Compared with the traditional compound library screening, the synthesis and screening cost of peptide library is relatively low, which makes this method more attractive in economic benefits.
• Efficient and fast: the screening can be completed in about 3-4 weeks at the earliest, saving customers time and cost.

FAQ

1. What is phage display peptide library screening?

Phage display peptide library screening is a technique used to identify peptides that bind specifically to a target molecule. This process involves exposing a target to a vast library of phage-displayed peptides and selecting those that have the highest affinity for the target.

2. What types of targets can be screened using your service?

We can screen a variety of targets, including proteins, antibodies, small molecules, DNA, RNA, and even whole cells or tissues. This flexibility makes phage display peptide library screening suitable for numerous research and development applications.

3. What applications can benefit from phage display peptide library screening?

• Drug discovery and development
• Identification of binding motifs
• Epitope mapping
• Vaccine development
• Biomarker discovery
• Study of protein-protein interactions

4. What information do I need to provide for the screening service?

To begin the screening process, you need to provide detailed information about your target, including its nature, concentration, and any specific binding conditions or buffers that should be used. If you have specific preferences for the screening protocol, please let us know.

5. What methods do you use to ensure the success of the screening?

We utilize a combination of techniques, including biopanning and next-generation sequencing, to ensure the successful identification of high-affinity binders. Our protocols are optimized for specificity and sensitivity to yield the best possible results.

6. What deliverables can I expect at the end of the screening process?

Upon completion of the screening, you will receive a comprehensive report that includes:

• Detailed methodology of the screening process
• Data analysis and results
• Sequences of the identified peptides
• Binding affinity data
• Recommendations for further validation and characterization

7. Can you assist with the validation and characterization of identified peptides?

Yes, we offer additional services to validate and characterize the identified peptides. This includes affinity measurements, kinetic studies, and structural analysis to confirm the binding properties and potential applications of the peptides.

8. What are the costs associated with phage display peptide library screening?

The cost of the screening service depends on the complexity of the target and the specific requirements of your project. Please contact our sales team to request a detailed quote based on your needs.

9. How do I place an order or request a quote?

To place an order or request a quote, please contact our sales team via email or phone. Alternatively, you can fill out the inquiry form on our website with details about your project, and we will get back to you with a tailored proposal.

Reference

1. Zhang, K., et al. The screening of therapeutic peptides for anti-inflammation through phage display technology. International Journal of Molecular Sciences. 2022, 23(15): 8554.