Random Peptide Library

Creative Peptides has a fast and high-throughput parallel peptide synthesis platform. We are committed to providing high-quality random peptide library services according to customer needs. Our synthetic random peptide library provides a flexible choice from crude products to 98% purity.

Introduction

The design of random peptide library is designed to replace selected amino acid residues simultaneously and randomly with 20 natural amino acids through shotgun approach. Our scientists can also design random libraries with specific physical and chemical properties to produce random peptide libraries with defined sizes and properties for target identification and drug discovery. Random peptide libraries excited by natural toxins or bioactive peptide have attracted much attention because of their great advantages as screening resources and physicochemical and pharmacological properties.

In order to prepare a random peptide library, random sequences are bound to specific positions in the scaffold (e.g., fibronectin, protein A, and Ankyrin, or Lipocalin). All of these proteins are soluble and thermostable, and the yield of recombinant expression is very high. Similar to minimized antibodies, initially selected proteins are usually further adjusted through engineering called "maturity" to improve their properties, such as affinity and specificity.

Applications

• Peptide sequence optimization
• Ligand and drug discovery
• Identify the target
• Enhance the activity of peptides
• Predict antibody structural epitope
• Elucidate potential alternatives for enhanced peptide activity

Our services

• Design of various random peptide libraries
• Calculate the sequence list of peptide library
• A large number of peptides can be synthesized by in situ peptide synthesis and chemical conjugation
• Provide suggestions for choosing the most effective peptide library design for your specific application
• Comprehensive modifications

Advantages

• Fast library synthesis, reliable delivery time (2-3 weeks)
• The purity is about 70% to > 90%
• QC reports of RP-HPLC, MS, and COA are provided for each peptide product
• No third pary outsourcing
• Directly lyophilized in 96 tube-plates or in individual vials

References

1. Oldenburg, K. R., Loganathan, D., Goldstein, I. J., Schultz, P. G., & Gallop, M. A. (1992). Peptide ligands for a sugar-binding protein isolated from a random peptide library. Proceedings of the National Academy of Sciences, 89(12), 5393-5397.
2. Scott, J. K., & Craig, L. (1994). Random peptide libraries. Current Opinion in Biotechnology, 5(1), 40-48.
3. Matsubara, T., Hiura, Y., Kawahito, O., Yasuzawa, M., & Kawashiro, K. (2003). Selection of novel structural zinc sites from a random peptide library. FEBS letters, 555(2), 317-321.