Overlapping Peptide Library

At Creative Peptides, we have been designing, synthesizing and applying peptide library technology for many years. We provide overlapping peptide library services that will be tailored to specific research issues.

Introduction

The design of overlapping peptide library is mainly used for whole protein scanning. The head-to-tail peptide matrix is a common method for screening linear or continuous epitopes. The design of this peptide library is mainly determined by two key parameters, peptide length and offset number. The design is gradually intercepted from the N-terminal to C-terminal of the target protein, there is likely to be a orphan peptide. We generally transfer one or more amino acids from the N-terminal to maintain the same length.

Choosing the appropriate step and peptide length can reduce the experimental cost and maximize the value of the data. The step is usually designed as a third of the peptide length. Long peptides contain more epitopes, but such peptides are generally difficult to synthesize and contain fewer peptides in the library. Short peptides are easier to synthesize and cheaper, but have fewer epitopes per peptide.

Application

• Identification of antigenic peptide sequence.
• Screening enzyme substrates.
• Identification of T cell epitope.
• Localized antibody epitope.
• Screening the binding sites of target proteins.

Services

Creative Peptides's overlapping peptide library can be designed according to customers' requirements and future experimental purposes, and provide personal services such as peptide library design consultation and peptide library design guidance according to customers' practical problems.

Advantage

• The purity of the crude peptide library is more than 30%. The purity of the crude product can also be as high as 80%, which mainly depends on the characteristics of the peptide sequence itself.
• Peptide library pure products, according to customer needs, we can provide different levels of purity: > 70%, > 80, or even 98%.
• An independent platform for peptide library synthesis.
• Custom overlapping peptide library in all sizes and formats, ranging from a small set of peptides to libraries of 1,000's of different species.
• The peptides synthesized in each channel are packaged separately to ensure no cross contamination.
• Provide HPLC purification report and MS test report.
• Experienced experts provide design help free of charge.

References

1. Reese, G., Ayuso, R., Leong-Kee, S. M., Plante, M. J., & Lehrer, S. B. (2001). Characterization and identification of allergen epitopes: recombinant peptide libraries and synthetic, overlapping peptides. Journal of Chromatography B: Biomedical Sciences and Applications, 756(1-2), 157-163.
2. Xu, Y., Wasnik, S., Baylink, D. J., Berumen, E. C., & Tang, X. (2017). Overlapping peptide library to map Qa-1 epitopes in a protein. JoVE (Journal of Visualized Experiments), (130), e56401.
3. Hu, H., Li, L., Kao, R. Y., Kou, B., Wang, Z., Zhang, L., ... & Liu, G. (2005). Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library. Journal of combinatorial chemistry, 7(5), 648-656.