Proteases Substrate, Fluorogenic
Proteases Substrate, Fluorogenic
This is a fluorescent generic fluorogenic substrate for proteases such as trypsin, cathepsin B and L, Abs/Em=501/527. The colorless (Z-Arg)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments.
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| CAT# | X21157 |
| Sequence | One Letter Code: (Z-R)2Rh110 • 2HCl
Three Letter Code: (Z-Arg)2Rh110•2HCl |
| Storage | -20°C desiccated and protected from light |
Solubility | DMSO |
References | 1. Halangk W, et al. (1997). Trypsinogen activation in rat pancreatic acinar cells hyperstimulated by caerulein. Biochim Biophys Acta 1362, 243-51,
2. Banati RB, et al. (1993). Detection of lysosomal cysteine proteinases in microglia: flow cytometric measurement and histochemical localization of cathepsin B and L. Glia 7, 183-91,
3. Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54. |
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