MMP Colorimetric Substrate I
MMP Colorimetric Substrate I
An exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.
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| CAT# | X21175 |
| M.W/Mr. | 655.9 |
| Sequence | One Letter Code: Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
Three Letter Code: Ac-Pro-Leu-Gly-SCH[CH2CH(CH3)2]-CO-Leu-Gly-OC2H5 |
| Storage | -20°C |
References | 1. Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985);
2. Weingarten, H. et al. Biochem. 24, 6730 (1985). |
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