Cathepsin D and E FRET Substrate
Cathepsin D and E FRET Substrate
An internally quenched fluorogenic substrate (Ab/Em =328/393 nm) for cathepsins D and E and not for B, H or L, obtained from the hepatopancreas (liver) of the Japanese common squid (Todarodes pacificus). The cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence and is used in screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue cell extracts.
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| CAT# | F15001 |
| M.W/Mr. | 1756.1 |
| Sequence | One Letter Code: Mca-GKPILFFRLK(Dnp)-r-NH2
Three Letter Code: 7-Methoxycoumarin-4-Acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 |
| Storage | -20°C |
References | Yasuda, Y. et al. J. Biochem. 125, 1137 (1999). |
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