Peptide ELISA

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Peptide ELISA is a special type of enzyme immunoassay (EIA) that allows the quantitative analysis of a target molecule using antibodies. Antibodies are used to specifically detect analytes (e.g. peptides, proteins, antibodies, small molecules). Enzymes (e.g., horseradish peroxidase (HRP)) directly or indirectly couple to the antibody to provide the assay and possibly amplify the signal.

Peptide ELISA provides a simple, reliable and cost-effective method to quantitatively analyze one or more antigens in a variety of sample types such as cell lysates, tissue lysates or serum. It is a popular technique for research and diagnostic samples and can be used as a single sample assay or high-throughput screening method.

The experimental principle is based on specific binding of antibody/antigen, which allows not only the quantitative analysis of specific proteins, but also the study of molecular interactions or other properties. An antibody against a target is coupled to a detection enzyme. Upon addition of the substrate, this enzyme catalyzes the production of a colored product from the substrate. The concentration of the antigen in the sample is then known by spectrophotometric measurement. Peptide ELISA enables analysis and screening on amino acid sequence level, e.g. mapping of epitopes or definition of protein interaction sites, thus providing much more information than conventional ELISA.

ELISA manipulation is a task that requires great care. The operator should have a good understanding of the experiment and be very clear about the expected results before performing the ELISA. Most of the reasons for failures are caused by operational errors. Peptide ELISA is considered a gold standard for the quantitative analysis of biological samples due to the specificity of the antibody and the simplicity of the assay. It can be used for antibody epitope mapping, immune profiling, determination of antibody titers, analysis of protein-protein interactions, analysis of enzymatic reactions, validation of microarray results.

Benefits of Peptide ELISA

• High batch-to-batch reproducibility
• Economic production of tailored ELISA plates
• Easy-to-use and compatible with standard ELISA protocols and equipment
• Generation of quantitative results
• High sensitivity